Quick question: describe the use of microorganisms in the food industry, with reference to yoghurt and single cell protein.
Thanks in advance
1.Two species of
bacteria Lactobacillus bulgaricus and
Lactococcus thermophilus in approximately equal proportions, are used to make yoghurt.
NOTE : You need
not know the names of the bacteria. I just mentioned it
Commercial producers pasteurize and homogenize the milk before adding the starter. After stirring, the mixture is then incubated for 3-6 hours at 40-450C. At this temperature the two bacteria have a mutually stimulating effect on one another.
Protolytic enzymes from L. bulgaricus break down milk proteins into peptides. These stimulate the growth of L. thermophilus which, in turn, produce formic acid and carbon dioxide, growth stimulants for L. bulgaricus. As the incubation proceeds, L. bulgaricus converts the lactose to lactic acid and the pH falls to 4.2-4.4 which leads to the coagulation of proteins by lactic acid and the thickening of the yoghurt.
Further processing involves the addition of flavour, colour, fruit pulp and heat treatment to kill off any bacteria.
2.The most common
single-celled protein is
yeast. It is used in the manufacture of bread and beer.
The principal dry ingredients used in bread-making are wheat flour, salt, sugar, ascorbic acid, and the yeast Saccharomyces cerevisiae. Water or milk is added to produce a thick, sticky dough which is repeatedly folded or kneaded, to create nuclei for gas production and expansion. The mixed dough is rested, folded, and moulded and in a humid atmosphere at 34-350C, it is left for 10 minutes to ‘prove’.
During the process of ‘proving’, fermentation of sugars in the dough, catalysed by enzymes from the yeast cells, produces carbon dioxide. As this gas cannot escapes from the sticky mass, it fills the gas nuclei and causes the dough to “rise” or increase in volume. This process is sometimes is called ‘leavening’. The overall effects of leavening are to make the dough lighter, more digestible, and of better flavour following from the production of organic acids, alcohols and esters by yeast cells. The role of enzymes in the process are summarized as follows:
maltase
a) Maltose ---------------› glucose
(in flour) (from yeast)
invertase
b) Sucrose-----------------› glucose + fructose
(added) (from yeast)
zymase
c) Glucose + fructose----------------› alcohol + carbon dioxide
(from yeast)
The yeast fermented bread have better flavour and texture as compared to the one prepared by using baking powder therefore in the commercial production of bread, Baker’s yeast is used. The living cells of aerobically grown Saccharomyces cerevisiae are collectively referred to as baker’s yeast.
Baker’s yeast is commercially available either as a dried powder i.e. dry yeast with about 95% dry weight or in the form of cakes (about 25-30% dry weight). In recent years, some workers have reported the development of genetically engineered strains of Saccharomyces cerevisiae with improved fermentation properties.
NOTE : Again you need
not know the names of the yeast.
Hope it helps