guys provide best answer for this effect of pH on activity of enzyme p6 m
marking scheme says1 same amount / volume / concentration of amylase;
2 same amount / volume / concentration of starch;
3 same temperature;
4 vary pH, at least 3 for a range ;5 reasonable suggested detail to obtain a different pH, ideally use of buffer;
6 regular timing for testing;
7 repetition;
8 3 named items of apparatus selected; my answer is this is it good
take 2 cm3 of 1% amylase in5 test tubes.prepare 5 different solutions of pH say 2 4 5 6 7 8 in 5 different test tubes label them a to e maintain using buffer solution then take 5cm3 of starch solution in 5 different test tubes at last take 5cm3 of iodine solution i dropper pipette add one solution of amylase in one particular pH solution and add it to one starch solution and suddenly on the timmer oberve time taken to diappear the blue color repeat with all solutions of ph
repeat using different ranges of pH
