IGCSE/GCSE/O & A Level/IB/University Student Forum

Qualification => Subject Doubts => GCE AS & A2 Level => Sciences => Topic started by: XaM!! on May 29, 2010, 01:50:54 pm

Title: Enzyme practical
Post by: XaM!! on May 29, 2010, 01:50:54 pm

Hey has anyone done enzyme related praciticals? if yes then plz tell me what happens in it.

Title: Re: Enzyme practical
Post by: XaM!! on May 29, 2010, 05:40:41 pm

Any1?

Title: Re: Enzyme practical
Post by: hesho21 on May 29, 2010, 11:43:18 pm

See the only problem with ure post is that u didnt give us an example so we can explain it. If you just mention an experiment , I would be glad to help :D

Title: Re: Enzyme practical
Post by: nid404 on May 30, 2010, 05:32:07 am

Usually it's breakdown of H2O2 using catalase. Either enzyme or substrate conc is varied....and then you note the bubbles per min or measure the volume of gas evolved per min.

Title: Re: Enzyme practical
Post by: XaM!! on May 30, 2010, 06:19:56 am

Hey nid Thanks.. buh can u give me any example frm paper... iv heard catalase is coming in practical.

Title: Re: Enzyme practical
Post by: nid404 on May 30, 2010, 06:39:31 am

yup you got that right :P

I'll have to browse through the papers...I'll look though

Title: Re: Enzyme practical
Post by: XaM!! on May 30, 2010, 07:42:08 am

Ahan Thanks a lot.............

Title: Re: Enzyme practical
Post by: hesho21 on May 30, 2010, 06:07:54 pm

really catalase is coming ? guys u know if blood cells or the gas exchange system are coming?

Title: Re: Enzyme practical
Post by: XaM!! on May 30, 2010, 07:16:00 pm

Wat? blood cells? no no no... v cant even see thm. lol

Title: Re: Enzyme practical
Post by: hesho21 on May 30, 2010, 09:48:12 pm

lol u haven't seen any pictures of red blood cells or lymphocytes b4? :D

Title: Re: Enzyme practical
Post by: XaM!! on May 31, 2010, 03:06:20 am

obviously i hav buh u cant c thm in a light microscope...

Title: Re: Enzyme practical
Post by: hesho21 on May 31, 2010, 07:53:42 am

dude sry my bad , how can I miss this :D

Title: Re: Enzyme practical
Post by: thecandydoll on May 31, 2010, 02:09:29 pm

So any hints about practicals.
Anyway.
I wanted to know the answers to these questions:
What are your sources of error in enzyme catalysed reactions.
And how would you improve the experiment (:
Thank you in advancee

Title: Re: Enzyme practical
Post by: Chingoo on May 31, 2010, 04:27:11 pm

Quote from: XaM!! on May 31, 2010, 03:06:20 am

obviously i hav buh u cant c thm in a light microscope...

o.0 Of course you can. You must have some wacko teachers.

Title: Re: Enzyme practical
Post by: XaM!! on May 31, 2010, 04:46:36 pm

you cant see red blood cell...

Title: Re: Enzyme practical
Post by: Chingoo on May 31, 2010, 04:58:35 pm

Quote from: XaM!! on May 31, 2010, 04:46:36 pm

you cant see red blood cell...

You can. You can see platelets, all kinds of WBCs too. Sorry to burst your bubble, but that's true. When you light microscope can spot a nucleus and even the mitochondria, how the hell can't it spot RBC which is7 times an average mitochondrion's size? See the logic.