IGCSE/GCSE/O & A Level/IB/University Student Forum

Qualification => Subject Doubts => GCE AS & A2 Level => Sciences => Topic started by: sgd on May 17, 2010, 01:41:53 pm

Title: I need help in Sucrose Dilution biology practical CIE :)
Post by: sgd on May 17, 2010, 01:41:53 pm

How to dilute a sucrose soln from 10% to 1% and vice versa
Also, how to dilute from 10% to 5% and vice versa

Desperate need of help :)

Title: Re: I need help in Sucrose Dilution biology practical CIE :)
Post by: ~Mimi~ on May 18, 2010, 07:40:49 am

its simple.
Calculations for serial dilution:
c?v?=c?v?

c1 is concentration of  current solution you will use
v1 is volume of current solution used
c2 is concentration of new solution you want to make
v2 is volume of new solution you want to make
So, for example I want to make 10 cubic cm of 5% sucrose solution by using 10% sucrose solution. I need to know how much of 10% to use.
10V? = 5 x 10
V? = 5 ml

So you will take 5 ml of the 10% sucrose solution given and add 5 ml of water so that it makes a final volume of 10 cubic cm. Then shake gently before you do anything with the 5% solution.

hope i helped.