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ALL CIE BIOLOGY DOUBTS HERE !!!

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Vampire-Love4ever:
Explain the relationship between action spectrum and absorption spectrum.

**RoRo**:
How do we know what type of inhibitor the chemical/substance that is added is?
Example: Q3 a iii) on this paper: http://www.xtremepapers.me/CIE/International%20A%20And%20AS%20Level/9700%20-%20Biology/9700_w10_qp_23.pdf
How do we know it's a non-competitive/irreversible inhibitor?

& someone please answer my previous questions:

--- Quote ---Just a couple of questions here,

Specimen paper 2001, Q3 a iii) - What's the answer?
Specimen paper 2001, Q4 is about genetic engineering, is that part of our syllabus?
Specimen paper 2001, Q3 b) - What's the answer?

Can anyone please send me the link to the MS of this paper?
--- End quote ---

Master_Key:

--- Quote from: The Secret on May 16, 2011, 08:08:47 am ---How do we know what type of inhibitor the chemical/substance that is added is?
Example: Q3 a iii) on this paper: http://www.xtremepapers.me/CIE/International%20A%20And%20AS%20Level/9700%20-%20Biology/9700_w10_qp_23.pdf
How do we know it's a non-competitive/irreversible inhibitor?

& someone please answer my previous questions:

--- Quote ---Just a couple of questions here,

Specimen paper 2001, Q3 a iii) - What's the answer?
Specimen paper 2001, Q4 is about genetic engineering, is that part of our syllabus?
Specimen paper 2001, Q3 b) - What's the answer?

Can anyone please send me the link to the MS of this paper?

--- End quote ---

Cu2+ ions are not the shape of sucrose so it is non-competitive.
--- End quote ---

Amelia:

--- Quote from: The Secret on May 16, 2011, 08:08:47 am ---How do we know what type of inhibitor the chemical/substance that is added is?
Example: Q3 a iii) on this paper: http://www.xtremepapers.me/CIE/International%20A%20And%20AS%20Level/9700%20-%20Biology/9700_w10_qp_23.pdf
How do we know it's a non-competitive/irreversible inhibitor?

--- End quote ---

Like MK said, Cu+ ions are not the shape of the sucrose so they do not attach themselves in the active-sites of the enzymes but elsewhere in the enzyme molecule. They alter the shape of the enzyme in such a way that the active site can no longer accomodate the substrate. As the substrate & inhibitor molecules attach on different parts of the enzyme, they are not competing for the same sites (non-competivite). Also, metal ions break the disulphide bonds(that help maintain the shape of the enzyme molecule). This causes the enzyme molecule's structure to be irreversibly altered.


--- Quote ---& someone please answer my previous questions:

--- End quote ---

Attach the questions please.  :-\

Amelia:

--- Quote from: Vampire-Love4ever on May 16, 2011, 12:15:27 am ---Explain the relationship between action spectrum and absorption spectrum.

--- End quote ---

An action spectrum is a graph showing the effectiveness of different wavelengths of light in stimulating the process being investigated. An absorption spectrum is a graph of the relative amounts of lights absorbed at different wavelengths by a pigment.

An action spectrum for photosynthesis is shown together with an absorption spectrum for the combined photosynthetic pigments.

http://5e.plantphys.net/images/ch07/wt0701c.png

NOte the close similarity, which indicates that the pigments, cholorophylls in particular, are those responsible for absorption of light in photosynthesis.

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