Doubts attached!
counting the number of cells on the grid under view gives th no. of cells per 0.04mm
2 (since its th area of the grid) If less than half of the cell is in the grid, its not counted. The area 0.04 is multiplied by the depth 0.1mm to give the volume of the grid. this gives 0.004mm
3. Now jus count the number of cells under view and divide by the volume (which is identical to multiplying by 250 cuz 1/0.004 = 250) to find the no. of cells per mm
3 - mention doing repeats n etc...
next part - it was mentioned in the begining tht th original number of cells was 3thousand. in the table where the mean is - the column heading is thousands of cells per mm
3 of culture - so ur mean of 6.2 is actually 6.2 thousand --- now for calculating th percentage increase just follow wat they said takinf th final number as ur mean value and the original number as 3 - thts it